Applied Nanophysics -> Publications by Prof. Fernando D. Stefani, Ph.D.
Aleksandra K. Adamczyk, Teun A. P. M. Huijben, Karol Kolataj, Fangjia Zhu, Rodolphe Marie, Fernando D. Stefani, Guillermo P Acuna
Towards full control of molecular exciton energy transfer via FRET in DNA origami assemblies
Submitted – Leer paper
Morgane Loretan, Mariano Barella,*, Nathan Fuchs, Samet Kocabey, Karol Kołątaj, Fernando D. Stefani, Guillermo P. Acuna
Direct single-molecule detection and super-resolution imaging with a low-cost portable smartphone-based microscope
Submitted - bioRxiv (2024) – Leer paper
Santiago Sosa, Alan M. Szalai, Lucía F. Lopez, Juan Manuel Prieto, Cecilia Zaza, Aleksandra K. Adamczyk, Hernan R. Bonomi, Marcelo Marti, Guillermo P. Acuna, Fernando A. Goldbaum, Fernando D. Stefani
Monitoring conformations of a single molecule inside a protein cavity under physiologically compatible conditions
Submitted
José Wojnacki, Gonzalo Quassollo, Martín D. Bordenave, Nicolás Unsain, Gaby F. Martínez, Alan M. Szalai, Olivier Pertz, Gregg . Gundersen, Francesca Bartolini, Fernando D. Stefani, Alfredo Cáceres, Mariano Bisbal
Dual spatio-temporal regulation of axon growth and microtubule dynamics by RhoA signaling pathways
Journal of Cell Science 137 (2024) jcs261970 - Leer paper
Piotr Zdańkowski, Lucía F Lopez, Florencia Edorna, Guillermo P Acuna, Fernando D Stefani
Single Molecule Localization and Nanoscopy Through Sequential Structured Illumination
Book chapter in Super-Resolution Microscopy for Material Science, CRC Press, p. 133-155 – Leer paper
Luciana P. Martinez, María Cristina Mina Villarreal, Cecilia Zaza, Mariano Barella, Guillermo Acuna, Fernando D. Stefani, Ianina L. Violi, Julian Gargiulo.
Thermometries for Single Nanoparticles Heated with Light
ACS Sensors 9 (2024) 1049-1064 – Leer paper
Cole, Fiona; Zähringer, Jonas; Bohlen, Johann; Schröder, Tim; Steiner, Florian; Pfeiffer, Martina; Schüler, Patrick; Stefani, Fernando; Tinnefeld, Philip.
Super-resolved FRET and co-tracking in pMINFLUX.
Nature Photonics (2024), 18, pages 478–484 . Leer paper
Julian Gargiulo, Matias Herran, Ianina L. Violi, Ana Sousa-Castillo, Luciana P. Martinez, Simone Ezendam, Mariano Barella, Helene Giesler, Roland Grzeschik, Sebastian Schlücker, Stefan A. Maier, Fernando D. Stefani, Emiliano Cortes
Impact of bimetallic interface design on heat generation in plasmonic Au/Pd nanostructures studied by single-particle thermometry
Nature Communications 14 (2023) 3813 – Leer paper
Fernando D. Stefani
Tracking nanoscopic motion with minima of light
Nature Photonics 17 (2023) 552–553 – Leer paper
Lucía F. Lopez, Luciano A. Masullo, Alan M. Szalai, Florencia Edorna, Florencia D. Choque, Fernando Caprile, Fernando D. Stefani. “Optimization and characterization of toroidal foci for super-resolution fluorescence microscopy: a tutorial”
Journal of the Optical Society of America B 40 (2023) C103-C110. DOI: 10.1364/JOSAB.482413. Leer publicación
Luciana P. Martinez, Santiago Poklepovich-Caride, Julian Gargiulo, Eduardo D. Martínez, Fernando D. Stefani, Paula C. Angelomé, Ianina L. Violi. “Optical Printing of Single Au Nanostars”. Nano Letters 23 (2023) 2703-2709. DOI: 10.1021/acs.nanolett.2c05109 Ver publicación
Cecilia Zaza, Germán Chiarelli, Ludovit P. Zweifel, Mauricio Pilo-Pais, Evangelos Sisamakis, Fernando D. Stefani, Guillermo P. Acuna. “Super-resolved FRET imaging by confocal fluorescence-lifetime single-molecule localization microscopy”. Small Methods (2023) 2201565. DOI: 10.1002/smtd.202201565. Ver publicación
Fangjia Zhu, María Sanz-Paz, Antonio I. Fernández-Domínguez, Xiaolu Zhuo, Luis M. Liz-Marzán, Fernando D. Stefani, Mauricio Pilo-Pais, and Guillermo P. Acuña
“DNA-Templated Ultracompact Optical Antennas for Unidirectional Single-Molecule Emission”
Nano Letters 20 (2022) 6402–6408
Luciano A. Masullo, Alan M. Szalai, Lucía F. Lopez, Mauricio Pilo-Pais, Guillermo P. Acuna, and Fernando D. Stefani
“An alternative to MINFLUX that enables nanometre resolution in a confocal microscope”
Light: Science & Applications 11 (2022) 199
Luciana P. Martinez, Julian Gargiulo, Mariano Barella, Ianina L. Violi, Fernando D. Stefani
"Fine tuning the optical properties of single Au nanoparticles by plasmon-driven growth in closed-loop control"
Advanced Optical Materials 10 (2022) 2102724
Luciano A. Masullo, Fernando D. Stefani
“Multiphoton single-molecule localization by sequential excitation with light minima”
Light: Science & Applications 11 (2022) 70
Fernando Caprile, Luciano A. Masullo, Fernando D. Stefani
“PyFocus – a Python package for vectorial calculations of focused optical fields under realistic conditions. Application to toroidal foci”
Computer Physics Communications 275 (2022) 108315
Ianina L. Violi, Luciana P. Martinez, Mariano Barella, Cecilia Zaza, Lukáš Chvátal, Pavel Zemánek, Marina V. Gutiérrez, María Y. Paredes, Alberto F. Scarpettini, Jorge Olmos-Trigo, Valeria R. Pais, Iván Díaz Nóblega, Emiliano Cortes, Juan José Sáenz, Andrea V. Bragas, Julian Gargiulo, Fernando D. Stefani
“Challenges on Optical Printing of Colloidal Nanoparticles”
Journal of Chemical Physics 156 (2022) 034201
Luciano A. Masullo, Lucía F. Lopez, Fernando D. Stefani
“A common framework for single-molecule localization using sequential structured illumination”
Biophysical Reports 2 (2022) 100036
Luciano A. Masullo, Alan M. Szalai, Lucía F. Lopez, Fernando D. Stefani
“Fluorescence nanoscopy at the sub-10 nm scale”
Biophysical Reviews 13 (2022) 1101-1112
Alan M. Szalai, Cecilia Zaza, Fernando D. Stefani
“Super-resolution FRET measurements”
Nanoscale 13 (2021) 18421-18433
Rodrigo A. Ponzio, Ramiro M. Spada, Ana B. Wendel, M. Virginia Forcone, Fernando D. Stefani, Carlos A. Chesta, Rodrigo E. Palacios
“Exciton diffusion, antenna effect and quenching defects in superficially dye doped conjugated polymer nanoparticles"
Journal of Physical Chemistry C 125 (2021) 23299-23312
Kristina Hübner, Himanshu Joshi, Aleksei Aksimentiev, Fernando D. Stefani, Philip Tinnefeld, and Guillermo P. Acuna
"Determining the In-Plane Orientation and Binding Mode of Single Florescent Dyes in DNA Origami Structures"
ACS Nano 15,3 (2021) 5109-5117
Alan M. Szalai, Bruno Siarry, Jerónimo Lukin, Sebastián Giusti, Nicolás Unsain, Alfredo Cáceres, Florian Stelner, Philip Tinnefeld, Damián Refojo, Thomas M. Jovin, Fernando D. Stefani.
"Super-resolution Imaging of Energy Transfer by Intensity-Based STED-FRET"
Nano Letters 21 (2021) 2296-2303
Luciano A. Masullo, Florian Steiner, Jonas Zähringer, Lucía F. Lopez, Johann Bohlen, Lars Richter, Fiona Cole, Philip Tinnefeld, Fernando D. Stefani
“Pulsed Interleaved MINFLUX”
Nano Letters 21 2021 - 840-846
Alan Szalai, Bruno Siarry, Jerónimo Lukin, David J. Williamson, Nicolás Unsain, Raquel Becerra, Damián Refojo, Alfredo Cáceres, Mauricio Pilo-Pais, Dylan M. Owen, Guillermo Acuna, Sabrina Simoncelli, Fernando D. Stefani
“Three-dimensional total-internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules”
Nature Communications – 12 (2021) 517
Mariano Barella, Ianina L. Violi, Julian Gargiulo, Luciana P. Martinez, Florian Goschin, Victoria Guglielmotti, Diego Pallarola, Sebastian Schlücker, Mauricio Pilo-Pais, Guillermo P. Acuna, Stefan A. Maier, Emiliano Cortes, Fernando D. Stefani
“In Situ Photothermal Response of Single Gold Nanoparticles Through Hyperspectral Imaging Anti-Stokes Thermometry”
ACS Nano 15 (2020) 2458-2467
Alan M Szalai, Lucía F López, Miguel Ángel Morales-Vásquez, Fernando D Stefani, Pedro F Aramendia
“Analysis of sparse molecular distributions in fibrous arrangements based on the distance to the first neighbor in single molecule localization microscopy”
Nanoscale 12 (2020) 9495–9506
Annette M. Vogl , Lilian Phu, Raquel Becerra, Sebastian A. Giusti, Erik Verschueren, Trent B. Hinkle, Martín D. Bordenave, Max Adrian, Amy Heidersbach, Patricio Yankilevich, Fernando D. Stefani , Wolfgang Wurst, Casper C. Hoogenraad, Donald S. Kirkpatrick Damian Refojo and Morgan Sheng , “Global site-specific neddylation profiling reveals that NEDDylated cofilin regulates actin dynamics”
Nature Structural & Molecular Biology, Vol 27 , February 2020 , 210–220
Gaby f. Martínez, Nahir G. Gazal, Gonzalo Quassollo, Alan M. Szalai, Esther Del Cid-Pellitero, Thomas M. Durcan, Edward A. Fon, Mariano Bisbal, Fernando D. Stefani , Nicolas Unsain, “Quantitative expansion microscopy for the characterization of the spectrin periodic skeleton of axons using fluorescence microscopy”,
Scientific Reports (2020) 10:2917
Santiago Sosa, Andrés H. Rossi, Alan M. Szalai, Sebastián Klinke, Jimena Rinaldi, Ana Farías, Paula M. Berguer, Alejandro D. Nadra, Fernando D. Stefani, Fernando A. Goldbaum, Hernán R. Bonomi , “Asymmetric bifunctional protein nanoparticles through redesign of self-assembly”
Nanoescale Advances. 2019,1,1833
Kristina Hübner, Mauricio Pilo-Pais, Florian Selbach, Tim Liedl, Philip Tinnefeld*, Fernando D. Stefani*, Guillermo P. Acuna*
"Directing Single-Molecule Emission with DNA Origami-Assembled Optical Antennas"
Nano Letters 19 (2019) 6629-6634
Cecilia Zaza, Ianina L. Violi, Julián Gargiulo, Germán Chiarelli, Ludmilla Schumacher, Jurij Jakobi, Jorge Olmos-Trigo, Emiliano Cortes, Matthias König, Stephan Barcikowski, Sebastian Schlücker, Juan José Sáenz, Stefan A. Maier, and Fernando D. Stefani
"Size-Selective Optical Printing of Silicon Nanoparticles through Their Dipolar Magnetic Resonance"
ACS Photonics 6 (2019) 815-822
Alan M. Szalai, Natalia G. Armando, Federico M. Barabas, Fernando D. Stefani, Luciana Giordano, Sara E. Bari, Claudio N. Cavasotto, Susana Silberstein*, Pedro F. Aramendía*
"A fluorescence nanoscopy marker for corticotropin-releasing hormone type 1 receptor: computer design, synthesis, signaling effects, super-resolved fluorescence imaging, and in situ affinity constant in cells"
Physical Chemistry Chemical Physics 20 (2018) 29212-29220
Yanina D. Álvarez, Jesica V. Pellegrotti, Fernando D. Stefani
Book chapter: “Gold Nanoparticles as Nucleation Centers for Amyloid Fibrillation”
In: F. Santamaria, X. Peralta (eds) “Use of Nanoparticles in Neuroscience”. Neuromethods 135 (2018) 269-291. Humana Press, New York
Nicolás Unsain, Martin D. Bordenave, Gaby F. Martinez, Sami Jalil, Catalina von Bilderling, Federico Barabas, Luciano A. Masullo, Aaron D. Johnstone, Phil A. Barker, Mariano Bisbal, Fernando D. Stefani, Alfredo Cáceres
“Remodeling of the Actin/Spectrin Membrane-associated Periodic Skeleton, Growth Cone Collapse and F-Actin Decrease during Axonal Degeneration”
Scientific Reports 8 (2018) 3007
Federico M. Barabas, Luciano A. Masullo, Martín D. Bordenave, Sebastián A. Giusti, Nicolás Unsain, Damián Refojo, Alfredo Cáceres, Fernando D. Stefani
“Automated quantification of protein periodic nanostructures in fluorescence nanoscopy images: abundance and regularity of neuronal spectrin membrane-associated skeleton”
Scientific Reports 7 (2017) 16029
Julián Gargiulo, Ianina L. Violi, Santiago Cerrota, LukášChvátal, Emiliano Cortés, Eduardo M. Perassi, Fernando Diaz, Pavel Zemánek, Fernando D. Stefani
“Accuracy and Mechanistic Details of Optical Printing of Single Au and Ag Nanoparticles”
ACS Nano 11 (2017) 9678–968
Julian Gargiulo, Thomas Brick, Ianina L. Violi, Facundo C. Herrera, Toshihiko Shibanuma, Pablo Albella, Felix G. Requejo, Emiliano Cortés, Stefan A. Maier, Fernando D. Stefani
“Understanding and Reducing Photothermal Forces for the Fabrication of Au Nanoparticle Dimers by Optical Printing”
Nano Letters 17 (2017) 5747–5755
M. Raab, C. Vietz, F. D. Stefani, G. P. Acuna, P. Tinnefeld
“Shifting molecular localization by plasmonic coupling in a single-molecule mirage”
Nature Communications 8 (2017) 13966
F. Balzarotti, Y. Eilers, K. C. Gwosch, A. H. Gynnå, V. Westphal, F. D. Stefani, J. Elf, S. W. Hell
“Nanometer resolution imaging and tracking of fluorescent molecules with minimal photon fluxes”
Science 355 (2017) 606-612
F. M. Barabas, L. A. Masullo, F. D. Stefani
"Tormenta: An open source Python-powered control software for camera based optical microscopy"
Review of Scientific Instruments 87 (2016) 126103(1)–126103(3)
J. Gargiulo, S. Cerrota, E. Cortés, I. L. Violi, F. D. Stefani
“Connecting metallic nanoparticles by optical printing”
Nano Letters 16 (2016) ASAP - DOI: 10.1021/acs.nanolett.5b04542 pdf
Martín D Bordenave, Francisco Balzarotti, Fernando D Stefani, Stefan W Hell
“STED nanoscopy with wavelengths at the emission maximum”
Journal of Physics D 49 (2016) 365102 pdf
Jesica Pellegrotti, Guillermo Acuña, Anastasiya Puchkova, Phil Holzmeister, Andreas Gietl, Birka Lalkens, Fernando Stefani and Philip Tinnefeld
“Controlled reduction of photobleaching in DNA origami – gold nanoparticle hybrids.”
Nano Letters 14 (2014) 2831-2836 pdf
D. Brinks, R. Hildner, E. M. H. P. van Dijk, F. D. Stefani, J. B. Nieder, J. Hernando and N. F. van Hulst
“Ultrafast dynamics of single molecules”
Chemical Society Reviews 43 (2014) 2476-2491 pdf
Y. D. Álvarez, J. A. Fauerbach, J. V. Pellegrotti, T. M. Jovin, E. A. Jares-Erijman, F. D. Stefani
“Influence of gold nanoparticles on the kinetics of alpha-synuclein aggregation”
Nano Letters 12 (2013) 6156-6163 pdf
F. Balzarotti, F. D. Stefani
“Plasmonics Meets Far-Field Optical Nanoscopy”
ACS Nano 6 (2012) 4580-4584 pdf
G. P. Acuna, M. Bucher, I. H. Stein, Ch. Steinhauer, A. Kuzyk, P. Holzmeister, R. Schreiber, A. Moroz, F. D. Stefani, T. Liedl, F. C. Simmel, P. Tinnefeld
“Distance Dependence of Single-Fluorophore Quenching by Gold Nanoparticles Studied on DNA Origami”
ACS Nano 6 (2012) 3189-3195 pdf
S. R. Kirchner, A. Ohlinger, T. Pfeiffer, A. S. Urban, F. D. Stefani, A. Deak, A. A. Lutich, J. Feldmann
“Membrane composition of jetted lipid vesicles: a Raman spectroscopy study”
Journal of Biophotonics 5 (2012) 40-46 pdf
D. Brinks, R. Hildner, F. D. Stefani, N. F. van Hulst
“Beating spatio-temporal coupling: implications for pulse shaping and coherent control experiments”
Optics Express 19 (2011) 26486-26499 pdf
E. A. Coronado, E. R. Encina, F.D. Stefani
“Optical Properties of Metallic Nanoparticles: manipulating light, heat and forces at the nanoscale”
Nanoscale 3 (2011) 4042-4059 pdf
D. Brinks, R. Hildner, F. D. Stefani, N. F. van Hulst
“Coherent control of single molecules at room temperature”
Faraday Discussions 153 (2011) 51-60 pdf
T.H. Taminiau, F. D. Stefani, N. F. van Hulst
“Optical nanorod antennas as cavities for dipolar emitters: evolution of sub- and super-radiant modes”
Nano Letters 11 (2011) 1020-1024 pdf
R. Hildner, D. Brinks, F. D. Stefani, N. F. van Hulst
“Electronic Coherences and Vibrational Wave Packets in Single Molecules Studied with Femtosecond Phase-Controlled Spectroscopy”
PCCP 13 (2011) 1888-1894 pdf
A. S. Urban, A. A. Lutich, F.D. Stefani, J. Feldmann
“Laser Printing Single Gold Nanoparticles”
Nano Letters 10 (2010) 4794-4798 pdf
S. K. Dondapati, T. K. Sau, C. Hrelescu, T. A. Klar, F.D. Stefani, J. Feldmann
“Label-free Biosensing Based on Single Gold Nanostars as Plasmonic Transducers”
ACS Nano 4 (2010) 6318-6322 pdf
H. Ba, J. Rodríguez-Fernández, F.D. Stefani, J. Feldmann
“Immobilization of Gold Nanoparticles on Living Cell Membranes upon Controlled Lipid Binding”
Nano Letters 10 (2010) 3006-3012 pdf
D. Brinks, F. D. Stefani, F. Kulzer, R. Hildner, T. H. Taminiau, Y. Avlasevich, K. Müllen & N. F. van Hulst
“Visualizing and controlling vibrational wavepackets of single molecules”
Nature 465 (2010) 905-908 pdf
A.A. Lutich, A. Pöschl, G. Jiang, A. S. Susha, A. L. Rogach, F. D. Stefani, J. Feldmann
“Efficient energy transfer in layered hybrid organic/inorganic nanocomposites: a dual function of semiconductor nanocrystals”
Applied Physics Letters 96 (2010) 083109 pdf
G. Jiang, A. S. Susha, A. A. Lutich, F. D. Stefani, A. L. Rogach , J. Feldmann
“Cascaded Two‐Level FRET from Conjugated Polymer/Quantum Dot Complexes for DNA Hybridization Detection”
ACS Nano 3 (2009) 4127-4131 pdf
S. Mayilo, M. A. Kloster, M. Wunderlich, A. Lutich, T. A. Klar, A. Nichtl, K. Kürzinger, F. D. Stefani, J. Feldmann
“Long‐range fluorescence quenching by gold nanoparticles in a sandwich immunoassay for cardiac troponin T”
Nano Letters 9 (2009) 4558‐4563 pdf
M. Stemmler, F. D. Stefani, S. Bernhardt, R. E. Bauer, M. Kreiter, K. Müllen, W. Knoll
“One‐Pot Preparation of Dendrimer−Gold Nanoparticle Hybrids in a Dipolar Aprotic Solvent”
Langmuir 25 (2009) 12425-12428 pdf
